Destruxin B (Synonyms: 黑僵菌素 B)

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白、同位素标记物，专注于信号通路和疾病研究领域。
Destruxin B  (Synonyms: 黑僵菌素 B) 纯度: 99.86%

Destruxin B 是从昆虫病原真菌中分离的绿僵菌 (Metarhizium anisopliae)，是一种具有杀虫和抗癌活性的环肽。 Destruxin B 通过 Bcl-2 家族依赖的线粒体途径在人类非小细胞肺癌细胞中诱导细胞凋亡。Destruxin B 显著激活 caspase-3，并在体外和体内通过 caspase 介导的细胞凋亡减少肿瘤细胞增殖。

Destruxin B (Synonyms: 黑僵菌素 B)

Destruxin B Chemical Structure

CAS No. : 2503-26-6

规格 价格 是否有货 数量
1 mg ￥7800 In-stock
5 mg   询价  
10 mg   询价  

* Please select Quantity before adding items.

Destruxin B 相关产品

•同靶点产品:

•同靶点蛋白产品:

生物活性

Destruxin B, isolated from entomopathogenic fungus Metarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities. Destruxin B induces apoptosis via a Bcl-2 Family-dependent mitochondrial pathway in human nonsmall cell lung cancer cells[1]. Destruxin B significantly activates caspase-3 and reduces tumor cell proliferation through caspase-mediated apoptosis, not only in vitro but also in vivo[2].

IC50 & Target

Bcl-2

 

Caspase-3

 

体外研究
(In Vitro)

Destruxin B (1-30 μM; 48 hours) inhibits cell proliferation of H1299 cells and A549 cells, with IC50s of 4.1 μM and 4.9 μM, respectively[1].
Activation of mitochondria-dependent caspase cascade plays an important role in Destruxin B (1-30 μM; 48 hours) induced apoptotic cell death in A549 cells[1].
Destruxin B (1.25-20.00 μM; 72 hours) treatment significantly inhibits HT-29 human colorectal cancer cells viability in time- and dose-dependent manners[2].
Destruxin B (10 or 20 μM; 12 and 24 hours) treatment markedly increases levels of the proapoptotic protein PUMA and reduces levels of antiapoptotic proteins Mcl-1 of A549 cells in a concentration- and time-dependent manner [1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Destruxin B 相关抗体:

Cell Proliferation Assay[1]

Cell Line: H1299 cells
Concentration: 1, 5, 10, 20, 30 μM
Incubation Time: 48  hours
Result: Inhibited H1299 cells proliferation of H1299 cells with an IC50 of 4.1 μM.

Apoptosis Analysis[1]

Cell Line: Lung adenocarcinoma A549 cells
Concentration: 1, 5, 10, 20, 30 μM
Incubation Time: 48  hours
Result: Induces caspase-dependent Lung adenocarcinoma A549 cells death.

Cell Viability Assay[2]

Cell Line: HT-29 human colorectal cancer cells
Concentration: 1.25, 2.50, 5.00, 10.00 and 20.00 μM
Incubation Time: 72 hours
Result: The IC50s at 24 h, 48 h and 72h were determined as 14.97, 2.00 and 0.67 μM, respectively.

Western Blot Analysis[1]

Cell Line: A549 cells
Concentration: 10 or 20 μM
Incubation Time: 12 and 24  hours
Result: Regulates Mcl-1 and PUMA.

体内研究
(In Vivo)

Destruxin B (DB) ( injection; 0.6-15 mg/kg/day for 6 weeks) attenuates the tumor growth in time- and dose-dependent manners[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic female nude mice (BALB/cAnN.Cg-Foxn1nu/CrlNarl), approximately 4-5 weeks old on arrival[2]
Dosage: Low dose (0.6 mg/kg), medium dose (3 mg/kg), high dose (15 mg/kg)
Administration: Injection; daily; for 6 weeks
Result: Low dose, medium dose and high dose indicated a reduction of 23.9%, 33.4% and 55.8% in mean tumor size, respectively.

分子量

621.81

Formula

C32H55N5O7
CAS 号

2503-26-6
性状

固体

颜色

White to off-white

中文名称

黑僵菌素 B
结构分类
  • Cyclopeptides
初始来源
  • 微生物

Metarhizium anisopliae
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
纯度 & 产品资料

纯度: 99.86%

Data Sheet (545 KB) SDS (251 KB)

COA (180 KB) RP-HPLC (275 KB)

产品使用指南 (1538 KB)

参考文献

  • [1]. Wu CC, et al. Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human NonsmallCell Lung Cancer Cells. Evid Based Complement Alternat Med. 2013;2013:548929.

    [2]. Lee YP, et al. In vitro and in vivo anticancer effects of destruxin B on human colorectal cancer. Anticancer Res. 2012 Jul;32(7):2735-45.  [Content Brief]

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务