Brassicasterol is a metabolite of Ergosterol and has cardiovascular protective effects. Brassicasterol exerts anticancer effects in prostate cancer through dual targeting of AKT and androgen receptor signaling pathways. Brassicasterol inhibits HSV-1 (IC₅₀=1.2 μM) and Mycobacterium tuberculosis. Brassicasterol also inhibits sterol δ 24-reductase, slowing the progression of atherosclerosis. Brassicasterol is also a cerebrospinal fluid biomarker for Alzheimer’s disease^{[1][2][3][4][5][6]}.

Brassicasterol (10 μM; 24 h) 下调二氢睾酮 (DHT) 诱导的 LNCaP 细胞中雄激素受体 (AR) 和前列腺特异性抗原 (PSA) 的表达^[4]。
Brassicasterol (50 μM; 48 h) 将 LNCaP 的细胞周期阻滞在亚 G1 期，并诱导细胞凋亡^[4]。
Brassicasterol (10 μM; 48 h) 还抑制 LNCaP 细胞迁移^[4]。
Brassicasterol (100 μg/mL; 48 h) 在 3D 类器官模型中，能够抑制 AKT，并对 AR 不依赖的癌症以及 AR 依赖的细胞其抑制效力^[4]。

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

398.66

C₂₈H₄₆O

474-67-9

固体

White to light yellow

油菜甾醇； 芜莆甾醇； 菜籽甾醇

• Steroids

• 动物

Hippocampus abdominalis

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Ethanol 中的溶解度 : 4.76 mg/mL (11.94 mM; 超声助溶)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

• 方案 一

  请依序添加每种溶剂： 10% EtOH    90% Corn Oil

  Solubility: ≥ 0.5 mg/mL (1.25 mM); 澄清溶液

  此方案可获得 ≥ 0.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。

  以 1 mL 工作液为例，取 100 μL 5.0 mg/mL 的澄清 EtOH 储备液加到 900 μL 玉米油中，混合均匀。

• [1]. Yasuharu Yazawa, et al. Inhibitory effect of ergosterol on bladder carcinogenesis is due to androgen signaling inhibition by brassicasterol, a metabolite of ergosterol. J Nat Med. 2020 Sep;74(4):680-688.  [Content Brief]

  [2]. Sherif T S Hassan. Brassicasterol with Dual Anti-Infective Properties against HSV-1 and Mycobacterium tuberculosis, and Cardiovascular Protective Effect: Nonclinical In Vitro and In Silico Assessments. Biomedicines. 2020 May 24;8(5):132.  [Content Brief]

  [3]. Yinzhu Xu, et al. Brassicasterol from Edible Aquacultural Hippocampus abdominalis Exerts an Anti-Cancer Effect by Dual-Targeting AKT and AR Signaling in Prostate Cancer. Biomedicines. 2020 Sep 22;8(9):370.  [Content Brief]

  [4]. Vanmierlo T, et al. The plant sterol brassicasterol as additional CSF biomarker in Alzheimer’s disease. Acta Psychiatr Scand. 2011 Sep;124(3):184-92.  [Content Brief]

  [5]. Fernández C, et al. Inhibition of cholesterol biosynthesis by Delta22-unsaturated phytosterols via competitive inhibition of sterol Delta24-reductase in mammalian cells. Biochem J. 2002 Aug 15;366(Pt 1):109-19.  [Content Brief]

  [6]. Tansley G, et al. Sterol lipid metabolism in down syndrome revisited: down syndrome is associated with a selective reduction in serum brassicasterol levels. Curr Gerontol Geriatr Res. 2012;2012:179318.  [Content Brief]