Swinholide A

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白、同位素标记物，专注于信号通路和疾病研究领域。
Swinholide A 

Swinholide A 是结合肌动蛋白的一种海洋聚酮化合物，可将肌动蛋白二聚化，Kd 约为 50 nM。 Swinholide A 是一种破坏海洋毒素的微丝，可稳定肌动蛋白二聚体并切断肌动蛋白丝。Swinholide A 破坏细胞的肌动蛋白细胞骨架。具有抗真菌 (antifungal)活性。

Swinholide A

Swinholide A Chemical Structure

CAS No. : 95927-67-6

规格 价格 是否有货 数量
10 μg 询价
10 μg ￥2439 询价

* Please select Quantity before adding items.

Swinholide A 相关产品

•同靶点产品:

•同靶点蛋白产品:

生物活性

Swinholide A is the actin-binding marine polyketide and dimerizes actin with the Kd of ~ 50 nM[1]. Swinholide A is a microfilament disrupting marine toxin that stabilizes actin dimers and severs actin filaments. Swinholide A disrupts the actin cytoskeleton of cells.Antifungal activity[2].

体外研究
(In Vitro)

Swinholide A, first isolated from the Okinawan marine sponge Theonella swinhoei, dimerizes actin[1].
Swinholide A, isolated from the marien sponge Theonella swinhoei, is highly cytotoxic to a variety of cancer cell lines[2].
Swinholide A disrupts the actin cytoskeleton of cells grown in culture, sequesters actin dimers in vitro in both polymerizing and non-polymerizing buffers with a binding stoichiometry of one swinholide A molecule per actin dimer, and rapidly severs F-actin in vitro with high cooperativity[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Swinholide A 相关抗体:

Cell Viability Assay[2]

Cell Line: Balb/c 3T3 and Swiss 3T3 cells
Concentration: 5-100 nM
Incubation Time: 1-24 h
Result: Exponentially growing cells exposed to 10 nM for 24 h became arborized with diffuse cytoplasmic staining and fluorescent punctate structures.
Partial cell retraction or arborization and diminution of microfilament bundles began after 2-4 h, with complete loss of stress fibers by 5-7 h at concentrations of 10-50 nM.
Caused rounding of cultured mouse embryo 3T3 fibroblast cells within 1 h at concentration of 80 nM.

分子量

1389.87

Formula

C78H132O20
CAS 号

95927-67-6
结构分类
  • Others
初始来源
  • 海洋天然产物
  • 海绵
  • 动物

Marine sponges
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
Data Sheet (540 KB) 产品使用指南 (1538 KB)

参考文献

  • [1]. Inji Shin,et al. Total Synthesis of Swinholide A: An Exposition in Hydrogen-Mediated C-C Bond Formation. J Am Chem Soc. 2016 Nov 2;138(43):14246-14249.  [Content Brief]

    [2]. M R Bubb, et al. Swinholide A is a microfilament disrupting marine toxin that stabilizes actin dimers and severs actin filaments. J Biol Chem. 1995 Feb 24;270(8):3463-6.  [Content Brief]

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务