The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.

 

Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

 

Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.

 

Discover more assay kits for enzyme activity measurement.

 

Content: (Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) Or

(Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser)

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: β-Glucanase/Lichenase

Assay Format: Spectrophotometer, Auto-analyser

Detection Method: Absorbance

Wavelength (nm): 400

Signal Response: Increase

Limit of Detection: (Malt β-glucanase) 4.3 x 10-4 U/mL Or

(Lichenase) 9.1 x 10-5 U/mL

Reproducibility (%): ~ 3%

Total Assay Time: (Malt β-glucanase) ~ 20 min Or

(Lichenase) ~ 10 min 

Application examples: Crude malt extracts, industrial enzyme preparations.

Method recognition: Novel method

 

Advantages

Very cost effective 

All reagents stable for > 2 years 

Specific for endo-1,3:1,4-β-glucanase/lichenase 

Simple, convenient, rapid assay 

Well suited to automation 

Malt flour standard and lichenase standard included

 

MBG4试剂含有单一底物，即4,6-O-亚苄基-2-氯-4-硝基苯基-β-（31-β-D-纤维三糖基葡糖苷）（BCNPBG4）。亚苄基缩醛基团阻止外作用水解酶如β-葡萄糖苷酶或纤维二水解酶的任何水解作用。

混合连接的β-葡聚糖酶（内切-1,3:1,4-β-葡聚糖蛋白酶）/地衣酶（EC 3.2.1.73）特异性地从该底物中释放2-氯-4-硝基苯酚（CNP）。CNP的释放速率与样品中的β-葡聚糖酶/地衣酶活性直接相关。终止反应并在加入Tris缓冲溶液（pH＝10.0）时形成酚酸酯颜色。

注意，底物不被β-葡萄糖苷酶或纤维二水解酶水解。底物可以被某些内切纤维素酶（例如木霉属）水解，但这不会导致吸光度的增加。

发现更多用于酶活性测量的检测试剂盒。

含量：（麦芽β-葡聚糖酶）100测定（手动）/400（自动分析仪）或

（Lichenase）100/200测定（手动）/330（自动分析仪）

运输温度：环境温度

储存温度：短期稳定性：2-8oC，

长期稳定性：参见单个组件标签

稳定性：在推荐的储存条件下超过2年

分析物：β-葡聚糖酶/Lichenase

测定形式：分光光度计、自动分析仪

检测方法：吸光度

波长（nm）：400

信号响应：增加

检测限：（麦芽β-葡聚糖酶）4.3 x 10-4 U/mL或

（Lichenase）9.1 x 10-5 U/mL

再现性（%）：~3%

总测定时间：（麦芽β-葡聚糖酶）~20分钟或

（Lichenase）~10分钟

应用实例：粗麦芽提取物、工业酶制剂。

方法识别：一种新颖的方法

优点

非常经济高效

所有试剂稳定超过2年

对内切-1,3:1,4-β-葡聚糖酶/地衣酶具有特异性

简单、方便、快速测定

非常适合自动化