The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).

 

New, improved procedure.

 

In the most recent development, a recombinant endo-levanase has been incorporated into the fructanase mixture, extending the use of the method to the measurement of levan-type fructans as are present in grasses such as timothy, cocksfoot, ryegrass and red fescue.

 

The method described in this booklet employs ultra-pure, recombinant enzymes and specifically measures fructans including inulin-type fructans from chicory, dahlia, jerusalem artichoke; highly branched fructans from onion and wheat stems and leaves; and levan-type fructans from pasture grasses such as timothy grass. The enzymes employed are completely devoid of contaminating enzymes active on β-glucan or gluco-oligosaccharides.

 

Content: 100 assays per kit

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: Fructan

Assay Format: Spectrophotometer

Detection Method: Absorbance

Wavelength (nm): 410

Signal Response: Increase

Linear Range: 2.3 to 55 µg of D-fructose or D-glucose per assay

Limit of Detection: 0.16 g/100 g

Total Assay Time: ~ 90 min

Application examples: Flours, infant formula, animal feed, pet foods, plant materials (e.g. onion), food products and other materials

Method recognition: AACC Method 32-32.01, AOAC Method 999.03, AOAC Method 2016.14, AOAC Method 2018.07 and CODEX Method Type III

 

Advantages

Very cost effective 

All kit reagents stable for > 2 years after preparation 

Unaffected by high sucrose / reducing sugar concentrations  

Fructan kits are only available from Megazyme 

Simple format 

Mega-Calc™ software tool is available from our website for hassle-free raw data processing 

Standard included

 

 

果聚糖检测试剂盒适用于植物提取物、动物饲料和含有淀粉、蔗糖和其他糖的食品中果聚糖的特异性测量。它用于三种已验证的果聚糖测定方法：AOAC方法999.03（食品）、AOAC方法2018.07（动物饲料）和AOAC方法2016.14（婴儿配方奶粉和成人营养品）。

新的、改进的程序。

在最新的发展中，一种重组的内切levanase已被掺入果聚糖酶混合物中，将该方法的应用扩展到测定存在于timothy、鸡爪、黑麦草和红羊茅等草中的levan-型果聚糖。

本手册中描述的方法采用超纯重组酶，并专门测量果聚糖，包括菊苣、大丽花、菊芋中的菊粉型果聚糖；来自洋葱和小麦茎和叶的高度分枝的果聚糖；以及来自牧草如提摩太草的levan型果聚糖。所用的酶完全没有对β-葡聚糖或低聚葡糖有活性的污染酶。

内容：每个试剂盒100个分析

运输温度：环境温度

储存温度：短期稳定性：2-8oC，

长期稳定性：参见单个组件标签

稳定性：在推荐的储存条件下超过2年

分析物：果聚糖

测定形式：分光光度计

检测方法：吸光度

波长（nm）：410

信号响应：增加

线性范围：每次测定2.3至55µg D-果糖或D-葡萄糖

检测限：0.16g/100g

总测定时间：~90分钟

应用示例：面粉、婴儿配方奶粉、动物饲料、宠物食品、植物材料（如洋葱）、食品和其他材料

方法识别：AACC方法32-32.01，AOAC方法999.03，AOAC方式2016.14，AOAC办法2018.07和CODEX方法类型III

优点

极具成本效益

所有试剂盒在制备后稳定2年以上

不受高蔗糖/还原糖浓度的影响

果聚糖试剂盒仅可从Megazyme获得

简单格式

Mega Calc™ 我们的网站上提供了软件工具，可以轻松处理原始数据

包含标准