爱尔兰Megazyme 淀粉总量检测试剂盒 K-TSTA-100A Total Starch Assay Kit (AA/AMG) 货号:MK-TSTA-100A 品牌:Megazyme


爱尔兰Megazyme 淀粉总量检测试剂盒 K-TSTA-100A

英文名:Total Starch Assay Kit (AA/AMG)

货号:MK-TSTA-100A

规格:100 assays (manual)

商品描述

 爱尔兰Megazyme 淀粉总量检测试剂盒 

英文名:Total Starch (AA/AMG) Assay Kit

货号:K-TSTA-100A

规格:100 assays per kit

分析物意义:主要的食品组分

Content: 50 assays / 100 assays

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: Total Starch

Assay Format: Spectrophotometer

Detection Method: Absorbance

Wavelength (nm): 510

Signal Response: Increase

Linear Range: 4 to 100 μg of D-glucose per assay

Limit of Detection: 0.18 g/100 g total starch “as is”

Total Assay Time: ~ 90 min

Application examples: Cereal flours, food products and other materials.

Method recognition: AACC Method 76-13.01, AOAC Method 996.11, ICC Standard Method No. 168 and RACI Standard Method

The Total Starch (AA/AMG) Assay Kit is used for the determination of total starch in cereal flours and food products.  AOAC Method 996.11, AACC Method 76-13.01.

 

This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0). 

 

含量:50次分析/100次分析

运输温度:环境温度

储存温度:短期稳定性:2-8oC,

长期稳定性:参见单个组件标签

稳定性:在推荐的储存条件下超过2年

分析物:总淀粉

测定形式:分光光度计

检测方法:吸光度

波长(nm):510

信号响应:增加

线性范围:每次测定4至100μg D-葡萄糖

检测限:0.18g/100g总淀粉“原样”

总测定时间:~90分钟

应用示例:谷物粉、食品和其他材料。

方法识别:AACC方法76-13.01、AOAC方法996.11、ICC标准方法168和RACI标准方法

总淀粉(AA/AMG)测定试剂盒用于测定谷物粉和食品中的总淀粉。AOAC方法996.11,AACC方法76-13.01。

 

该试剂盒现在含有一种改进的α-淀粉酶,可以在pH 5.0(以及pH 7.0)下进行淀粉酶孵育。

 

淀粉大致包含两种成分,直链淀粉主要是通过α-1,4-糖苷键连接的 D-葡聚糖,含有极少的 α-1,6 支链;而支链淀粉中葡萄糖分子之间除了以 α-1,4-糖苷键相连外,还有以 α-1,6-糖苷键相连的分支结构。淀粉的酶解法主要是将淀粉置于高温下,通过耐热α-淀粉酶进行糊化,以产生一系列直链和支链糊精,随后用淀粉葡糖苷酶(AMG)定量水解为葡萄糖。在 ATP 和 NADP+ 存在下,使用葡萄糖氧化酶/过氧化物酶 (GOPOD) 试剂或己糖激酶/磷酸葡萄糖脱氢酶检测释放的葡萄糖。在一系列成功研究的基础上,该方法被采用为 AOAC 方法 996.11、AACC国际方法 76-13.01 和 ICC 方法 168。
本试剂盒在 AOAC 方法 996.11 的基础上进行了适当的优化,从而提供了一个更加方便用户的淀粉检测方法,即快速总淀粉检测法
 

The Total Starch (AA/AMG) test kit is used for the measurement and analysis of total starch in cereal flours and food products. This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0).

Colourimetric method for the determination of Total Starch in
cereal products, feeds, foodstuffs and other materials

 

Principle:
(α-amylase, 100°C ± DMSO)
(1) Starch granules + H2O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose

(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Cereal flours, food products and other materials
Method recognition:
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard Method
No. 168), and RACI (Standard Method)

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 12 months after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

 

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