For the specific measurement of cellulase (endo-1,4-β-glucanase) in enzyme preparations and fermentation products.

Colourimetric method for the determination of endo-1,4-β-
glucanase (cellulase) in enzyme preparations and fermentation
products

Principle:
(endo-1,4-β-glucanase)
(1) Benzylidene-G₃-β-CNP + H₂O → Blocked-G_X + G_(3-X)-β-CNP

(thermostable β-glucosidase)
(2) G_(3-X)-β-CNP + H₂O → D-glucose + CNP

(alkaline solution)
(3) CNP → phenolate ion (yellow colour)
Note: CNP = 2-Chloro-4-nitrophenol

Kit size:  180 / 360 assays / 720 (auto-analyser)
Method:  Spectrophotometric at 400 nm
Total assay time: ~ 20 min
Detection lim-it:  0.05 U/mL
Application examples: 
Fermentation broths, industrial enzyme preparations, biofuels research
Method recognition:  Novel method
Note:  The analogous fluorimetric reagent (R-CELLF) 
 is also available with 10-fold greater sensitivity

Advantages

• Very cost effective
  
• All reagents stable for > 2 years after preparation
  
• Completely specific for cellulase (endo-1,4-glucanase). The substrate is not hydrolysed by β-glucosidase, cellbiohyrolase or any other enzymes tested
  
• Kinetic assays possible due to significant phenolate ion presence (and UV absorbance) at pH 5-6
  
• Simple format. Well suited to automation
  
• Standard included