The molecular weight of Poly-L-Ornithine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule.  This product’s molecular weight ranges from 70,000 to 150,000 Da.

 

Poly-L-Ornithine is supplied in a 50 ml amber bottle containing 5 mg of Poly-L-Ornithine. The product is ready-to-use after proper addition of cell culture water and sterile filtration. 

 

Parameter, Testing, and Method Poly-L-Ornithine #5172

Form Powder

Package Size 100 mg

Storage Temperature -20°C

Sterilization Method Non-Sterile

Molecular Weight 70,000 – 150,000 Da

Cell Attachment Assay Pass

Source Synthetic

Shelf Life Minimum of 6 months from date of receipt

Directions for Use

Use these recommendations as guidelines to

 

determine the optimal coating conditions for your culture system.  To maintain sterility, perform all operations in a laminar flow hood.

 

A typical working concentration is 0.1 mg/ml. If a different concentration is desired, transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using tissue culture grade water or PBS.

Sterile filter through a 0.22 micron filter. 

Add appropriate amount of diluted material to culture surface. Typically, 1 ml per 25 cm2is used. Rock gently to ensure uniform coating of culture surface.

Option A:

 

Allow to incubate for 60 minutes at room temperature, remove excess solution by aspiration.

Thoroughly rinse surface with tissue culture grade water and aspirate rinse water.

Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours. Coated cultureware can be stored for up to 1 week. Go to Step 9

Option B:

 

Allow to incubate overnight at room temperature, remove excess solution by aspiration.

Thoroughly rinse surface with tissue culture grade water and aspirate rinse water.

Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours. Coated cultureware can be stored for up to 1 week.

Introduce medium and cells to the culture surface.

Store remaining Poly-L-Ornithine solution at -10 to 30°C

 

Product Q & A

Is any animal derived material being used in the same production area of these adhesion peptide products?

This product is manufactured solely from synthetic materials and does not contain any raw materials or substances derived from animal origin.

 

Product References

References for Poly-L-Ornithine:

 

Chen, Eric YT, et al. "Activated charcoal composite biomaterial promotes human embryonic stem cell differentiation toward neuronal lineage." Journal of Biomedical Materials Research Part A 100.8 (2012): 2006-2017.

 

Boularaoui, Selwa Mokhtar, et al. "Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle." Journal of tissue engineering and regenerative medicine 12.2 (2018): e918-e936.

 

Ergin, Ekin, et al. "Time-resolved fluorescence resonance energy transfer [TR-FRET] assays for biochemical processes." Current pharmaceutical biotechnology 17.14 (2016): 1222-1230.