工具包包括大约184个测试样本和控制,包含2个微量滴定板。

Avian Leukosis Virus is a common naturally occurring neoplastic disease affecting chickens. AL is of great economic significance not only because of tumor production, but decreased egg production and increased mortality. Due to its long incubation period, AL affects mostly breeders and commercial egg layers. Enlargement of the bursa and liver may be detected by palpitation in advanced cases.1 Avian viruses in serum can be detected by the Enzyme Linked Immunosorbant Assay (ELISA). The levels of viral protein p27 in serum and egg albumen, as well as the disease status of entire flocks can be monitored using ELISA.
禽白血病病毒是一种常见的自然发生的肿瘤性疾病的影响鸡。铝不仅是重大的经济意义，因为肿瘤的生产，但产蛋量下降和死亡率的增加。由于其潜伏期长，铝主要影响种鸡和商品蛋鸡。的囊和肝脏肿大可能是由先进的检测血清中1例心悸。禽流感病毒可通过酶联免疫吸附试验（ELISA）检测。血清中蛋白的病毒蛋白p27的水平，以及整个羊群的疾病状态可监测使用ELISA。
Rabbit anti p27 antibody is bound to a microwell. Diluted sera or egg albumen is added to the microwell and incubated. Any p27 viral proteins present in the serum will bind to the -p27 on the microwell forming a p27 antibody-antigen complex. After washing away any unbound material, anti-p27 IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the p27 antibody-antigen complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 – 410 nm.
兔抗p27抗体绑定到微孔。稀释血清或蛋清加到微孔培养。任何病毒蛋白在血清蛋白会结合到p27在微孔形成p27抗原-抗体复合物。洗去任何未结合的物质后，抗p27抗体IgG碱性磷酸酶缀合物加入到微孔。共轭将绑定到p27抗原-抗体复合物。孵育后洗涤除去任何未结合的。酶的底物，然后添加到微孔。基板有碱性磷酸酶存在反应并形成黄色产物。在最后一步反应停止，固定的颜色。颜色的强度是在405 – 410 nm光度测量。