L-DABA (L-2,4-Diaminobutyric acid) is a week GABA transaminase inhibitor with an IC₅₀ of larger than 500 μM; exhibits antitumor activity in vivo and in vitro.

The tumor cells are irreversibly and totally damaged by incubation with 10 mM L-2,4-Diaminobutyric acid for 24 h at 37°C. The cell-destructive effect by L-DABA is probably due to an osmotic lysis induced by the non-saturated intracellular accumulation of L-DABA. The harmful effect of L-DABA could be abolished by concomitant incubation with L-alanine and L-methionine^[1]. Kinetic studies indicates that L-DABA is a non-linear, non-competitive inhibitor of GABA transaminase activity. The L-DABA-induced elevation of GABA levels parallels the inhibition of GABA transaminase activity^[2]. L-2,4-Diaminobutyric acid, an amino acid analogue, produceS a cytolytic effect with a human glioma cell line, SKMG-1, and normal human fibroblasts. The concentrations of L-DABA necessary to reduce the cell count to 50% of control following a 24-h incubation at 37°C are 12.5 mM for the human fibroblasts and 20 mM for the glioma cell line^[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Treatment with L-DABA results in 43.4% reduction of tumor growth^[1]. L-DABA is a more effective inhibitor of GABA transaminase in vivo than in vitro^[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

118.13

C₄H₁₀N₂O₂

1758-80-1

固体

White to off-white

L-2,4-二氨基丁酸

• Ketones, Aldehydes, Acids

• 内源性代谢物

Room temperature in continental US; may vary elsewhere.

H₂O 中的溶解度 : 1 mg/mL (8.47 mM; 超声助溶)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

• 方案 一

  请依序添加每种溶剂： PBS

  Solubility: 100 mg/mL (846.53 mM); 澄清溶液; 超声助溶

• [1]. Ronquist G, et al. Antitumor activity of L-2,4 diaminobuturic acid against mouse fibrosarcoma cells in vitro and in vivo. J Cancer Res Clin Oncol. 1980;96(3):259-68.  [Content Brief]

  [2]. Beart PM, et al. l-2,4-Diaminobutyric acid and the GABA system. Neurosci Lett. 1977 Jul;5(3-4):193-8.  [Content Brief]

  [3]. Panasci L, et al. The cytolytic effect of L-2,4 diaminobutyric acid with malignant glioma cells and fibroblasts. Cancer Chemother Pharmacol. 1988;21(2):143-4.  [Content Brief]

Mice: Male Sprague Dawley rats (150-200g) are used in the study. LDABA is dissolved in 09.% saline and diluted in appropriate medium. L-DABA is administered intraperitoneally at a dose of 764 mg/kg in a volume of 4 mL/kg in acute studies. Chronically treated rats receives daily intraperitoneally injections (2.5mM/kg in saline) for 3 days. Mice are sacrificed and the brain regions are dissected for analysis^[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Ronquist G, et al. Antitumor activity of L-2,4 diaminobuturic acid against mouse fibrosarcoma cells in vitro and in vivo. J Cancer Res Clin Oncol. 1980;96(3):259-68.  [Content Brief]

  [2]. Beart PM, et al. l-2,4-Diaminobutyric acid and the GABA system. Neurosci Lett. 1977 Jul;5(3-4):193-8.  [Content Brief]

  [3]. Panasci L, et al. The cytolytic effect of L-2,4 diaminobutyric acid with malignant glioma cells and fibroblasts. Cancer Chemother Pharmacol. 1988;21(2):143-4.  [Content Brief]