标签归档：daunorubicin

Daunorubicin (Synonyms: 柔红霉素; Daunomycin; RP 13057; Rubidomycin)

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Daunorubicin  (Synonyms: 柔红霉素; Daunomycin; RP 13057; Rubidomycin)

Daunorubicin (Daunomycin) 是一种拓扑异构酶 II (topoisomerase II) 抑制剂，具有有效的抗肿瘤活性。Daunorubicin 抑制 DNA 和 RNA 合成 (DNA and RNA synthesis)。Daunorubicin 是一种细胞毒素，可抑制癌细胞活力并诱导细胞凋亡 (apoptosis) 和坏死 (necrosis)。Daunorubicin 还是一种蒽环类抗生素。Daunorubicin 可用于研究感染和多种癌症，包括白血病、非霍奇金淋巴瘤、尤文氏肉瘤、维尔姆斯氏瘤。

Daunorubicin (Synonyms: 柔红霉素; Daunomycin; RP 13057; Rubidomycin)

Daunorubicin Chemical Structure

CAS No. : 20830-81-3

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生物活性

Daunorubicin (Daunomycin) is a topoisomerase II inhibitor with potent anti-tumor activity. Daunorubicin inhibits DNA and RNA synthesis. Daunorubicin is a cytotoxin that inhibits cancer cell viability and induces apoptosis and necrosis. Daunorubicin is also an anthracycline antibiotic. Daunorubicin can be used in the research of infection and variety of cancers, including leukemia, non-Hodgkin lymphomas, Ewing’s sarcoma, Wilms’ tumor[1][2][4][5].

IC50 & Target[1][2]

Topoisomerase II

 

Daunorubicins/Doxorubicins

 

体外研究
(In Vitro)

Daunorubicin (0-256 μg/mL, 30 min) inhibits DNA and RNA synthesis in sensitive and resistant Ehrlich ascites tumor cells[2].
Daunorubicin (7 nM-1.9 μM, 72 h) shows chemosensitivity in Molt-4 cells and L3.6 cells[3][4].
Daunorubicin (0.4 μM, 48 h) induces apoptotic and necrosis in L3.6 cells[4].
Daunorubicin (0.4 μM, 120 min) induces ROS generation in L3.6 cells[4].
Daunorubicin (2 μM, 24 h) induces autophagy in K562 cells (myeloid cell line)[6].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Daunorubicin 相关抗体:

Cell Viability Assay[3][4]

Cell Line: Molt-4 cells (a human T-lymphoblastic leukemia cell line), L3.6 cells (metastatic human pancreatic cell line)
Concentration: 7 nM-1.9 μM
Incubation Time: 72 h
Result: Inhibited cell viability with IC50 values of 40 nM (Molt-4) and 400 nM (L3.6).

Apoptosis Analysis[4]

Cell Line: L3.6 cells
Concentration: 0.4 μM
Incubation Time: 24 h, 48 h
Result: Induced necrosis without apoptosis at 24 h, induced both an apoptotic and extensive necrotic response at 48 h.

Western Blot Analysis[6]

Cell Line: K562 cells
Concentration: 2 μM
Incubation Time: 24 h
Result: Enabled the switch of LC3-I into LC3-II, accompanied with a significant increased expression level of LC3.

体内研究
(In Vivo)

Daunorubicin (intravenous injection, 3 mg/kg, three times at 48 h intervals.) produces cardiotoxicity and nephrotoxicity in rats[5].
Daunorubicin (intraperitoneal injection, 10 mg/kg) induces sister chromatid exchanges in mice[7].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats[5]
Dosage: 3 mg/kg
Administration: Intravenous injection, three times at 48 h intervals.
Result: Caused a significant increase in MDA (malondialdehyde) level in renal tissue, accompanied by a significant reduction in total GPx activity.
Increased urinary protein excretion, serum creatinine, and BUN level.

Clinical Trial

分子量

527.52

Formula

C27H29NO10
CAS 号

20830-81-3
中文名称

柔红霉素；红保霉素；红比霉素；红卫霉素；柔毛霉素；正定霉素；道诺霉素
结构分类
  • Quinones
  • Anthraquinones
  • Phenols
  • Polyphenols
初始来源
  • 微生物

Streptomyces peucetius and S. peucetius subsp. caesius
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
Data Sheet (548 KB) 产品使用指南 (1538 KB)

参考文献

  • [1]. Lehmann M, et al. Activity of topoisomerase inhibitors daunorubicin, idarubicin, and aclarubicin in the Drosophila Somatic Mutation and Recombination Test. Environ Mol Mutagen. 2004;43(4):250-7.  [Content Brief]

    [2]. Dano K, et al. Inhibition of DNA and RNA synthesis by daunorubicin in sensitive and resistant Ehrlich ascites tumor cells in vitro. Cancer Res. 1972 Jun;32(6):1307-14.  [Content Brief]

    [3]. Svensson SP, et al. Melanin inhibits cytotoxic effects of Doxorubicin and Daunorubicin in MOLT 4 cells. Pigment Cell Res. 2003 Aug;16(4):351-4  [Content Brief]

    [4]. Gervasoni JE Jr, et al. An effective in vitro antitumor response against human pancreatic carcinoma with paclitaxel and Daunorubicin by induction of both necrosis and apoptosis. Anticancer Res. 2004 Sep-Oct;24(5A):2617-26  [Content Brief]

    [5]. Arozal W, et al. Telmisartan prevents the progression of renal injury in daunorubicin rats with the alteration of angiotensin II and endothelin-1 receptor expression associated with its PPAR-γ agonist actions. Toxicology. 2011 Jan 11;279(1-3):91-9.  [Content Brief]

    [6]. Emeline Bollaert, et al. MiR-15a-5p Confers Chemoresistance in Acute Myeloid Leukemia by Inhibiting Autophagy Induced by Daunorubicin. Int J Mol Sci. 2021 May 13;22(10):5153.  [Content Brief]

    [7]. Cheng Wu, et al. Doxorubicin suppresses chondrocyte differentiation by stimulating ROS production. Eur J Pharm Sci. 2021 Dec 1;167:106013.  [Content Brief]

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