Deltonin, a steroidal saponin, isolated from Dioscorea zingiberensis, has antitumor activity; Deltonin inhibits ERK1/2 and AKT activation.

Deltonin inhibits ERK1/2 and AKT activation. Deltonin (0.5-8 μM) significantly inhibits the proliferation of several colon cancer cell lines including SW480, SW620, LOVO and C26, with IC₅₀s of 1.3, 1.29, 2.11 and 1.22 μM. Deltonin also causes G2-M phase arrest and induces apoptosis in C26 cells^[1].
Deltonin (0-5 μM) decreases the phosphorylation of AKT and ERK1/2 levels in MDA-MB-231 cells. Deltonin (0.5-8 μM) also dose-dependently inhibits the proliferation, and causes apoptosis with ROS production in MDA-MB-231 cells^[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Deltonin (20 and 40 mg/kg, p.o.) reduces the tumor growth of 37.7% and 56.7%, and causes a 50-day survival rate of 60% and 50%, respectively, in mice. Deltonin also inhibits tumor angiogenesis in mice^[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

885.04

C₄₅H₇₂O₁₇

55659-75-1

固体

White to off-white

三角叶薯蓣皂苷；三角叶薯蓣皂甙

• Steroids

• 植物
• 薯蓣科
• 盾叶薯蓣

• 内源性代谢物

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

DMSO 中的溶解度 : 50 mg/mL (56.49 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

• 方案 一

  请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

  Solubility: 2.5 mg/mL (2.82 mM); 悬浊液; 超声助溶

  此方案可获得 2.5 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

  生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。

• 方案 二

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

  Solubility: ≥ 2.5 mg/mL (2.82 mM); 澄清溶液

  此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

  20% SBE-β-CD in Saline 的配制（4°C，储存一周）：2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

• 方案 三

  请依序添加每种溶剂： 10% DMSO    90% Corn Oil

  Solubility: ≥ 2.5 mg/mL (2.82 mM); 澄清溶液

  此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. Tong QY, et al. Deltonin, a steroidal saponin, inhibits colon cancer cell growth in vitro and tumor growth in vivo via induction of apoptosis and antiangiogenesis. Cell Physiol Biochem. 2011;27(3-4):233-42.  [Content Brief]

  [2]. Zhang S, et al. Deltonin induces apoptosis in MDA‑MB‑231 human breast cancer cells via reactive oxygen species‑mediated mitochondrial dysfunction and ERK/AKT signaling pathways. Mol Med Rep. 2013 Mar;7(3):1038-44.  [Content Brief]

The cytotoxicity of Deltonin on colon cancer cells is determined by the MTT assay. Cells are seeded at a density of 3 × 10³ per well in 96-well plates. After incubation overnight, cells are treated with Deltonin in various concentrations (0.5, 1, 2, 4, 8 μM), and carrier DMSO (< 0.05%) is used as a control, 5 wells are included in each concentration. Every 24 h the absorbance at 570 nm is measured with SpectraMax M5, using wells without cells as blanks. All experiments are performed in triplicate. The concentration-and time-dependent curves of the Deltonin-treated colon cancer cell lines is generated as the % cell growth inhibition, using the following formula: % inhibition = (A570 of control - A570 of treated cells)/A570 of control cells × 100%. The 50% inhibiting concentration (IC₅₀) is calculated^[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Mice^[1]
Briefly, 5 × 10⁵ C26 cells are s.c. injected into 4- to 6-wk-old male BALB/c mice. The mice are randomly divided into four groups of 10 mice. One week after C26 implantation, the treatment groups receive their first doses of Deltonin dissolved in a vehicle solution of DMSO (< 0.1%) and diluted in saline solution. Briefly, Deltonin is given via oral administration to tumor-bearing mice at 10, 20 and 40 mg/kg every day for 3 weeks (between day 8 and 28). In parallel, the control group receives the vehicle (DMSO, < 0.1%) in saline solution. The same quantity of saline solution containing DMSO is used in these groups. General clinical observations of the mice, including determination of body weight and tumor growth, are made twice weekly. To determine tumor size, two perpendicular diameters of the tumor in centimeters is measured by calipers. Tumor volume is estimated using the formula 0.52 × a × b², where “a” is the long diameter and “b” is the short diameter. The mice are sacrificed when they become moribund, and the sacrifice date is recorded to calculate the survival time. To detect the apoptosis protein expression and microvessel density, tumor tissues are removed, fixed in 10% formalin or not and snap-frozen in liquid nitrogen immediately^[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Tong QY, et al. Deltonin, a steroidal saponin, inhibits colon cancer cell growth in vitro and tumor growth in vivo via induction of apoptosis and antiangiogenesis. Cell Physiol Biochem. 2011;27(3-4):233-42.  [Content Brief]

  [2]. Zhang S, et al. Deltonin induces apoptosis in MDA‑MB‑231 human breast cancer cells via reactive oxygen species‑mediated mitochondrial dysfunction and ERK/AKT signaling pathways. Mol Med Rep. 2013 Mar;7(3):1038-44.  [Content Brief]