3′-Deoxyuridine-5′-triphosphate trisodium (3′-dUTP trisodium) is a nucleotide analogue that inhibits DNA-dependent RNA polymerases I and II. 3′-Deoxyuridine-5′-triphosphate trisodium strongly and competitively inhibits the incorporations of UTP into RNA with a K_i value of 2.0 μM^[1].

3′-Deoxyuridine-5′-triphosphate (3′-dUTP) is synthesized starting from cordycepin in good yield. 3′-Deoxyuridine-5′-triphosphate strongly and competitively inhibits the incorporations of UTP into RNA by the RNA polymerases. 3′-Deoxyuridine-5′-triphosphate will be useful in studies at the molecular level on the relationship of template and substrate in RNA synthesis with chromatin, isolated nuclei or permeable cells, because it does not have any effect on poly (rA) synthesis^[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

534.09

C₉H₁₂N₂Na₃O₁₄P₃

固体

• Others

• 内源性代谢物

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

H₂O 中的溶解度 : 20 mg/mL (37.45 mM; 超声助溶)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

• 方案 一

  请依序添加每种溶剂： PBS

  Solubility: 50 mg/mL (93.62 mM); 澄清溶液; 超声助溶

• [1]. M Saneyoshi, et al. Inhibitory Effects of 3’deoxycytidine 5′-triphosphate and 3′-deoxyuridine 5′-triphosphate on DNA-dependent RNA Polymerases I and II Purified From Dictyostelium Discoideum Cells. Nucleic Acids Res. 1981 Jul 10;9(13):3129-38.  [Content Brief]