The D-Glucuronic/D-Galacturonic test kit is a simple, reliable and accurate method for the measurement and analysis of D-hexuronic acids (specifically D-glucuronic acid and D-galacturonic acid) in plant extracts, culture media/supernatants and other materials.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of D-Glucuronic Acid or
D-Galacturonic Acid in hydrolysates of plant material and
polysaccharides and other materials

Principle:
(Uronate dehydrogenase; UDH)
(1) D-Glucuronic acid + NAD⁺ + H₂O → D-glucarate + NADH + H⁺

(Uronate dehydrogenase; UDH)
(2) D-Galacturonic acid + NAD⁺ + H₂O → D-galactarate + NADH + H⁺

Kit size:  100 assays (manual) / 1000 (microplate) 
 / 1000 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time:  ~ 10 min at 25°C or ~ 5 min at 37°C
Detection limit:  ~ 17 mg/L
Application examples: 
Hydrolysates of plant material and polysaccharides and other
materials
Method recognition:  Novel method

Advantages

• Very cost effective
  
• All reagents stable for > 2 years during use
  
• Only test kit available
  
• Simple format
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Suitable for manual, microplate and auto-analyser formats