Geneva Biotech MultiBac™ MultiBac™ 货号：MultiBac™ 品牌：Geneva Biotech

 传统的分子生物学技术甚至现代合成生物学技术都不允许在一个真核细胞中方便地组装和拆卸共表达载体，从而产生多种蛋白质。multibac™表达系统使用DNA重组来实现昆虫细胞中多蛋白表达的杆状病毒载体的简单、高通量兼容构建。多蛋白共表达需要产生蛋白质复合物，如多价疫苗，或同一细胞中的多个单独蛋白质，如信号通路。多表达技术也是新兴合成生物学革命的基石。multibac™平台已用于产生完整的信号转导途径，如gpcr信号级联、激酶级联和蛋白酶级联。MULTIBAC™还被用于生产病毒样颗粒（VLP）和多种多蛋白复合物。杆状病毒表达载体系统（BEV），如multibac™是生产蛋白激酶、gpcrs和用于药物发现的离子通道的前线技术平台，是作为基因治疗传递载体的一个重要领域。以杆状病毒为载体的昆虫细胞表达也越来越多地用于生物制药的生产。MULTIBAC™转移载体是完全合成的，只包含功能性DNA，因此体积小且易于处理。multibac™杆状病毒基因组（DH10Embacy）正在不断开发，以去除有害/非必需基因（如v-cath和chia）和DNA重组热点，并包含一个集成的荧光蛋白表达盒，以便于监测病毒滴度。MULTIBAC™目前在全球1000多个学术和工业实验室使用。

Traditional molecular biology technologies and even modern synthetic biology technologies do not allow for convenient modular assembly and disassembly of co-expression vectors for production of multiple proteins in a single eukaryotic cell. The MultiBac™ expression system uses DNA recombination to allow for easy, high-throughput compatible construction of baculovirus vectors for multiprotein expression in insect cells. Multiprotein coexpression is required to produce protein complexes, such as multivalent vaccines, or multiple individual proteins in the same cell, such as for signaling pathways. Multiexpression technologies are also the cornerstone of the emerging synthetic biology revolution. The MultiBac™ platform has been used for production of whole intact signal transduction pathways such as GPCR signaling cascades, kinase cascades, and protease cascades. MultiBac™ has also been used for production of virus like particles (VLPs) and a wide variety of multiprotein complexes. Baculovirus Expression Vector Systems (BEVS) such as MultiBac™ are a front line technology platform for production of protein kinases, GPCRs and ion channels for drug discovery, and are a major area of interest as delivery vectors for gene therapy. Insect cell expression using baculovirus as a vector is also becoming increasingly used in manufacturing of biopharmceuticals. MultiBac™ transfer vectors are fully synthetic, comprising only functional DNA, and as a result are small and easy to handle. The MultiBac™ baculovirus genome (DH10EMBacY) is under continuous development to remove harmful/non-essential genes (e.g. v-cath and chiA) and DNA recombination hotspots, and contains an integrated fluorescent protein expression cassette to allow for easy monitoring of virus titer. MultiBac™ is presently used by over 1000 academic and industrial laboratories around the world.

Geneva Biotech MultiBac™ MultiBac™ 货号：MultiBac™ 品牌：Geneva Biotech

MULTIBAC™的工作原理：

1）将感兴趣的基因（gois）设计并克隆到multibc™受体和供体载体中。

2）匹配并混合您的GoI，然后（重新）将它们组合成一个结构

3）使用抗生素抗性标记的独特组合选择您的结构。

4）将整个GOIS组件转移到multibac™bacmid中。

5）选择并扩增含有multibc™bacmid的基因。

6）用纯化的multibac™bacmid导入昆虫细胞

7）昆虫细胞中蛋白质的表达

How MultiBac™ Works:

1) Design and clone gene(s) of interest (GOIs) into MultiBac™ acceptor and donor vectors

2) Match and mix your GOIs and then (re)combine them into one construct

3) Select your construct using unique combination of antibiotic resistance markers

4) Transfer the entire GOIs-assembly into the MultiBac™ bacmid

5) Select and amplify your gene-containing MultiBac™ bacmid

6) Transfect insect cells with purified MultiBac™ bacmid

7) Expression of protein(s) in insect cells

产品一览：

KITS

MultiBac™ Kit + Competent Cells1

CHEMICALLY COMPETENT CELLS

DH10EMBacY (12 x 100µl)

DH10MultiBac (12 x 100µl)

ig™ 10B (12 X 100µl)

PirHC (12 x 100µl)

PLASMIDS

pACEBac1 (5µg)

pACEBac2 (5µg)

pIDK (5µg)

pIDC (5µg)

pIDS (5µg)

DUAL PLASMIDS

pFL

pKL (5µg)

pSPL (5µg)>

pUCDM (5µg)

DUAL MAMMALIAN INSECT CELL PLASMIDS

pFL-IM (5µg)

pKL-IM (5µg)

pSPL-IM (5µg)

pUCDM-IM (5µg)