工具包包括大约184个测试样本和控制,包含2个微量滴定板。
 

Infectious Bronchitis Virus (IBV) is a clinically acute, highly contagious viral disease affecting chickens of all ages. IBV has an incubation period of only 24 to 72 hours. The disease affects the respiratory and urogenital tract of chickens. Young chickens exhibit acute respiratory disease signs and lesions in the trachea. Mortality may be as high as 100%1. Antibodies to avian viruses in serum can be detected by the Enzyme Linked Immunosorbant Assay (ELISA). The levels of IBV antibodies in serum, as well as the immune status of entire flocks can be monitored using ELISA.
传染性支气管炎病毒（IBV）是一种临床上急性，高度传染性的病毒性疾病，影响所有年龄的鸡。IBV也只有24至72小时的潜伏期。这种疾病影响呼吸道和泌尿生殖道的鸡。小鸡在气管具有急性呼吸道疾病的症状和病变。死亡率可高达100% 1。血清中的禽流感病毒抗体可通过酶联免疫吸附试验（ELISA）检测。血清中IBV抗体水平，以及整个鸡群免疫状态可监测使用ELISA。
Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-IBV antibodies present in the serum will bind to the IBV on the microwell forming an IBV antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the IBV antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 – 410 nm.
灭活抗原绑定到微孔。稀释的血清加入微孔培养。任何抗IBV血清中的抗体将结合在微孔形成IBV抗原抗体复合物的分离。洗去任何未结合的物质后，抗鸡IgG碱性磷酸酶缀合物加入到微孔。共轭将绑定到IBV抗原抗体复合物。孵育后洗涤除去任何未结合的。酶的底物，然后添加到微孔。基板有碱性磷酸酶存在反应并形成黄色产物。在最后一步反应停止，固定的颜色。颜色的强度是在405 – 410 nm光度测量。