标签归档：keramamine

Manzamine A hydrochloride (Synonyms: Keramamine A hydrochloride)

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上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白、同位素标记物，专注于信号通路和疾病研究领域。
Manzamine A hydrochloride  (Synonyms: Keramamine A hydrochloride) 纯度: 99.66%

Manzamine A hydrochloride 是一种具有口服活性的 β-carboline 生物碱，抑制 GSK-3β 和 CDK-5 的 IC50 值为 10.2 μM 和 1.5 μM。Manzamine A hydrochloride 靶向胰腺癌细胞的 vacuolar ATPases 并抑制自噬 (autophagy)。Manzamine A hydrochloride 具有抗疟和抗癌活性。Manzamine A hydrochloride 对 HSV-1 具有较强的活性。

Manzamine A hydrochloride (Synonyms: Keramamine A hydrochloride)

Manzamine A hydrochloride Chemical Structure

CAS No. : 104264-80-4

规格 价格 是否有货 数量
1 mg ￥2500 In-stock
5 mg ￥8000 In-stock
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Manzamine A hydrochloride 相关产品

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  • Bioactive Compound Library Plus

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生物活性

Manzamine A hydrochloride, an orally active beta-carboline alkaloid, inhibits specifically GSK-3β and CDK-5 with IC50s of 10.2 μM and 1.5 μM, respectively. Manzamine A hydrochloride targets vacuolar ATPases and inhibits autophagy in pancreatic cancer cells. Manzamine A hydrochloride has antimalarial and anticancer activities. Manzamine A hydrochloride also shows potent activity against HSV-1[1][2][3][4].

IC50 & Target[1][2][3]

GSK-3β

10.2 μM (IC50)

Plasmodium

 

CDK5

1.5 μM (IC50)

vacuolar ATPases

 

Malaria

 

HSV-1

 

体外研究
(In Vitro)

Manzamine A (5-50 µM, 18 h) hydrochloride decreases tau phosphorylation, measured with ELISA[1].
Manzamine A (10 µM) hydrochloride inhibits yeast S. cerevisiae growth by 30%[2].
Manzamine A hydrochloride displays a few enlarged vacuoles in yeast[2].
Manzamine A (2.5-10 µM, 24 h) hydrochloride increases acidity in pancreatic cancer cells and non-malignant Vero cells[2].
Manzamine A (1 µM, 24 h) hydrochloride inhibits HSV-1 infection in SIRC cells[4].
Manzamine A hydrochloride shows antimalarial activity with an IC50 of 8.0 nM (D6 clone) and 11 nM (W2 clone)[5].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Manzamine A hydrochloride 相关抗体:

Cell Viability Assay[4]

Cell Line: SIRC cell
Concentration: 0.1, 0.5, 1, 2, 3, 5, and 10 µM
Incubation Time: 72 h
Result: Inhibited SIRC cell viability with an IC50 of 5.6 µM.

体内研究
(In Vivo)

Manzamine A (50 and 100 mol/kg, p.o. or i.p.) hydrochloride inhibits the growth of the rodent malaria parasite Plasmodium berghei in infected mice[6].
Manzamine A (8 mg/kg, i.p., daily for 8 consecutive days) hydrochloride prolongs the survival of SW mice to 20 days[7].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Plasmodium berghei in infected mice[6]
Dosage: 50 or 100 mol/kg
Administration: Intraperitoneal injection (i.p.) or oral administration (p.o.)
Result: Inhibited the growth of the rodent malaria parasite Plasmodium berghei. Prolonged the survival of highly parasitaemic mice.

分子量

585.22

Formula

C36H45ClN4O
CAS 号

104264-80-4
性状

固体

颜色

Light yellow to brown

结构分类
  • Alkaloids
  • Pyridine Alkaloids
  • Alkaloids
  • Piperidine Alkaloids
  • Alkaloids
  • Indole Alkaloids
初始来源
  • 海洋天然产物
  • 海绵
  • 动物

sponge
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : 5.88 mg/mL (10.05 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7088 mL 8.5438 mL 17.0876 mL
5 mM 0.3418 mL 1.7088 mL 3.4175 mL
10 mM 0.1709 mL 0.8544 mL 1.7088 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

=

浓度

×

体积

×

分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×

体积 (start)

V1

=

浓度 (final)

C2

×

体积 (final)

V2

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动物溶解方案

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果
工作液所需浓度 : mg/mL

纯度 & 产品资料

纯度: 99.66%

Data Sheet (620 KB) SDS (252 KB)

COA (209 KB) RP-HPLC (256 KB)

产品使用指南 (1538 KB)

参考文献

  • [1]. Hamann M, et al. Glycogen synthase kinase-3 (GSK-3) inhibitory activity and structure-activity relationship (SAR) studies of the manzamine alkaloids. Potential for Alzheimer’s disease. J Nat Prod. 2007;70(9):1397-1405.  [Content Brief]

    [2]. Kallifatidis G, et al. The marine natural product manzamine A targets vacuolar ATPases and inhibits autophagy in pancreatic cancer cells [published correction appears in Mar Drugs. 2014;12(4):2305-7]. Mar Drugs. 2013;11(9):3500-3516. Published 2013 Sep 17.  [Content Brief]

    [3]. Winkler JD, et al. Antimalarial activity of a new family of analogues of manzamine A. Org Lett. 2006;8(12):2591-2594.  [Content Brief]

    [4]. Palem JR, et al. Manzamine A as a novel inhibitor of herpes simplex virus type-1 replication in cultured corneal cells. Planta Med. 2011;77(1):46-51.  [Content Brief]

    [5]. Wahba AE, et al. Structure-activity relationship studies of manzamine A: amidation of positions 6 and 8 of the beta-carboline moiety. Bioorg Med Chem. 2009 Nov 15;17(22):7775-82.  [Content Brief]

    [6]. Donia M, et al. Marine natural products and their potential applications as anti-infective agents. Lancet Infect Dis. 2003 Jun;3(6):338-48.  [Content Brief]

    [7]. El Sayed KA, et al. New manzamine alkaloids with potent activity against infectious diseases. J Am Chem Soc. 2001 Mar 7;123(9):1804-8.  [Content Brief]

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