每个套件包含大约184个测试样品加控制,包含2个微量滴定板。
 

Infectious laryngotracheitis (commonly known as ILT) is a viral respiratory disease of chickens centred on the trachea. ILT can cause serious production losses due to a combination of mortality and depressed egg production. This disease can manifest itself in a variety of forms ranging from severe to mild with the latter being increasingly encountered in developed poultry industries and appearing as a mucoid tracheitis, sinusitis and conjunctivitis coupled to unthriftiness and low mortality.
传染性喉气管炎（俗称ILT）是鸡的集中在气管的呼吸道疾病。ILT可造成严重的产量损失由于死亡率和郁闷的鸡蛋生产。本病可在各种各样的形式从严重到轻度与后者在发展养鸡业，为粘液性气管炎越来越多地遇到表现，鼻窦炎和结膜炎与挥霍浪费和低死亡率。
INTENDED USE
用途
ELI-LTI ELISA kit is used for quantitative detection of antibodies against Infectious Laryngotracheitis virus (ILT) in chicken serum and yolk.
eli-lti ELISA试剂盒用于抗传染性喉气管炎病毒（ILT）定量检测鸡血清和卵黄。
Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-ILT antibodies present in the serum will bind to the AIV on the microwell forming an AIV antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the ILT antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 – 410 nm with a 630-650 nm reference filter.
灭活抗原绑定到微孔。稀释的血清加入微孔培养。任何抗ILT抗体存在于血清中，将绑定到禽流感病毒在微孔形成的禽流感病毒的抗原抗体复合物。洗去任何未结合的物质后，抗鸡IgG碱性磷酸酶缀合物加入到微孔。共轭将绑定到ILT抗原抗体复合物。孵育后洗涤除去任何未结合的。酶的底物，然后添加到微孔。基板有碱性磷酸酶存在反应并形成黄色产物。在最后一步反应停止，固定的颜色。颜色的强度是光度测量在405 – 410 nm与630-650 nm参考滤波器。