每个套件包含大约184个测试样品加控制,包含2个微量滴定板。
 

Salmonella enteritidis (a paratyphoid Salmonella serotype) is a major food safety concern, primarily for the egg-laying industry. Possible sources in commercial layers include transmission from breeders, contaminated environments, infected rodents, and contaminated feed. Transmission to progeny from breeders is mainly through eggshell contamination, although, unlike other paratyphoid Salmonella , transovarial transmission may also occur. The NPIP now includes S enteritidis control measures in breeders, including depopulation of infected flocks, cleaning and disinfection of pullet and layer houses, extensive and improved rodent control programs, use of competitive exclusion products, vaccination, and proper handling and refrigeration of eggs.
肠炎沙门氏菌（甲型副伤寒沙门氏菌）是一种主要的食品安全问题，主要用于产蛋业。在商业层可能的来源包括传输来自饲养，污染环境，被感染的啮齿类动物，和被污染的饲料。从育种后代的传播主要是通过蛋壳污染，虽然，不像其他副伤寒沙门氏菌，还可能出现经卵传递。现在的美国的控制措施包括肠炎者，包括感染鸡群的灭绝，清洗和消毒成和层住宅，广泛提高鼠害控制程序，利用竞争排斥的产品，疫苗，和适当的处理和冷藏的鸡蛋。
Salmonella typhimurium , S enteritidis , and S heidelberg are among the most common salmonella infections in poultry, although infections may be produced by 10-20 different serotypes in the USA. Some species or strains are more pathogenic than others. The prevalence of other species varies widely by geographic location and season. Infected birds remain carriers. Although not common, clinical signs are sometimes seen in young birds. Mortality is most often limited to the first few weeks of age. Depression, poor growth, weakness, diarrhea, and dehydration are hallmarks of the disease, although these clinical signs are not distinctive. Lesions may include an enlarged liver with focal necrosis, unabsorbed yolk sac, enteritis with necrotic lesions in the mucosa, and cecal cores. Infections occasionally localize in the eye or synovial tissues.
鼠伤寒沙门氏菌，肠炎的，和海德堡是最常见的沙门氏菌感染的家禽中感染，虽然可以通过在美国，一些物种或不同血清型的菌株10-20产生比别人更多的致病性。其他物种的患病率有很大的地理位置和季节。受感染的鸟类保持运营商。虽然不常见，临床症状有时是在小鸟看到。死亡率通常是限于时代的最初几周。抑郁，生长不良，乏力，腹泻，脱水是一个巨大的疾病，虽然临床症状不明显。病变可能包括扩大肝局灶性坏死，未吸收的卵黄囊，在粘膜坏死病变肠炎，盲肠芯。感染偶尔定位在眼睛或滑膜组织。
INTENDED USE
用途
ELISalmonella E ELISA kit is used for quantitative detection of antibodies against Salmonella enteritidis in chicken serum and egg yolk.
elisalmonella E ELISA试剂盒用于抗肠炎沙门氏菌定量检测鸡血清和卵黄。
Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-SE antibodies present in the serum will bind to the SE on the microwell forming an SE antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the SE antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 – 410 nm with a 630-650 nm reference filter.
灭活抗原绑定到微孔。稀释的血清加入微孔培养。任何反硒血清中的抗体将结合到硒对硒微孔形成抗原抗体复合物。洗去任何未结合的物质后，抗鸡IgG碱性磷酸酶缀合物加入到微孔。共轭将绑定到这些抗原抗体复合物。孵育后洗涤除去任何未结合的。酶的底物，然后添加到微孔。基板有碱性磷酸酶存在反应并形成黄色产物。在最后一步反应停止，固定的颜色。颜色的强度是光度测量在405 – 410 nm与630-650 nm参考滤波器。