alt β-glucanase:                  
100 assays (manual format) / 400 assays (auto-analyser format)
Lichenase:                            
100 / 200 assays (manual format) / 330 assays (auto-analyser format)

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(3¹-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase. Mixed linkage 
β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g.Trichoderma sp.) but this does not result in an increase in absorbance.

Data calculators are located in the Documentation tab.

DESCRIPTION

Catalase (Aspergillus niger)

EC 1.11.1.16
CAS: 9001-05-2

Synonyms:
catalase; hydrogen-peroxide:hydrogen-peroxide oxidoreductase

Form:
In 3.2 M ammonium sulphate.
Supplied at ~ 18,000 U/mL.

Stability: 
> 2 years at 4^oC.

Specific activity:
~ 4,960 U/mg protein (using A₂₄₀ method) at pH 7.0 and 25^oC.

Unit definition:
One Unit of catalase activity will decompose 1 micromole of H₂O₂ per minute at pH 7.0 and 25^oC, while the H₂O₂ concentration falls from 10.3 mM to 9.2 mM. The rate of disappearance of H₂O₂ is followed by observing the rate of decrease in the absorbance at 
240 nm (A₂₄₀).

Specificity:
Decomposition of hydrogen peroxide into water and oxygen.

Applications:
For use in research.