每个套件包含大约184个测试样品加控制,包含2个微量滴定板。
 

Egg drop syndrome (EDS) is characterized by production of soft-shelled and shell-less eggs in apparently healthy birds. It has been recognized worldwide, except in the USA. It replicates well in chick kidney or chick-embryo liver cells and to a lesser degree in chick-embryo fibroblasts. The natural hosts for EDS virus are ducks and geese, and the disease has been described in Japanese quail ( Coturnix coturnix japonica ). Three types of disease are recognized in chickens. Classical EDS probably was due to contamination of a vaccine for Marek’s disease grown in duck-embryo fibroblasts and subsequent adaptation of the virus to chickens. Basic breeding stock was infected, and the virus was transmitted vertically through the egg. The virus often remained latent until the chick reached sexual maturity, when it was excreted in the eggs and droppings to infect susceptible contacts. Because the virus is vertically transmitted and is reactivated around peak egg production, there was an apparent breed and age susceptibility. However, all ages and breeds of chickens are susceptible, although the disease tends to be most severe in heavy broiler-breeders or brown egg producers. Arising from the classical form, endemic EDS has been reported in many areas and is usually seen in commercial egg producers. Flocks become infected at any stage in lay. Contaminated egg collection trays are one of the main forms of horizontal transmission, and outbreaks are often associated with a common egg-packing station.
减蛋综合征（EDS）的特点是生产的软壳、无壳蛋在表面健康的鸟。它已被世界公认，但在美国它复制在鸡肾或鸡胚肝细胞和鸡胚成纤维细胞中的一个较小的程度。EDS病毒的自然宿主是鸭和鹅，和疾病已在日本鹌鹑（鹌鹑）。三种疾病是公认的在鸡。古典EDS可能是由于对马立克氏病在鸭胚成纤维细胞和鸡的病毒随后的适应生长疫苗污染。基本种畜感染，和病毒是通过蛋垂直传播。病毒会潜伏在鸡达到性成熟，当它被排泄在鸡蛋和粪便感染易感接触。由于病毒的垂直传播和激活在高峰期产蛋，有一个明显的品种和年龄的易感性。然而，所有年龄和品种的鸡都易感，虽然这种疾病往往在重肉种鸡或棕色的鸡蛋生产商是最严重的。从经典的形式，特有的EDS已在许多领域的报道，通常是在商业鸡蛋生产商看到。羊群在任何阶段感染将成为。受污染的鸡蛋收集托盘是水平传播的主要形式，并爆发往往与普通蛋包装站。

用途
ELI-EDS ELISA kit is used for quantitative detection of antibodies against Egg Drop Syndrome virus (EDS) in chicken serum and yolk.
eli-eds ELISA试剂盒用于抗减蛋综合征（EDS）病毒定量检测鸡血清和卵黄。
Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-EDS antibodies present in the serum will bind to the EDS on the microwell forming an EDS antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the EDS antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 – 410 nm with a 630-650 nm reference filter.
灭活抗原绑定到微孔。稀释的血清加入微孔培养。任何抗EDS抗体存在于血清中，将绑定到的微孔形成抗原抗体复合物的EDS能谱仪。洗去任何未结合的物质后，抗鸡IgG碱性磷酸酶缀合物加入到微孔。共轭将绑定到EDS抗原抗体复合物。孵育后洗涤除去任何未结合的。酶的底物，然后添加到微孔。基板有碱性磷酸酶存在反应并形成黄色产物。在最后一步反应停止，固定的颜色。颜色的强度是光度测量在405 – 410 nm与630-650 nm参考滤波器。